This perfusion chamber is designed for oocyte two-electrode voltage clamp and patch clamp. The oocyte is confined in a cone shaped well to a fixed position. Since the volume of perfusion chamber's oocyte well is only 4 microliter, the solution change is almost instantaneous. The solution outlet is in an isolated chamber; the cell chamber does not experience water surface vibration due to the suction. The seal is thus unlikely to be broken by the solution change. The solution level is determined by a "water dam," which ensures a constant solution level.
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