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Perfusion chamber II
[Ne-Perfusion chamber II]

The perfusion chamber is designed for oocyte excised patch and tissue culture cells. Two 3-barrel perfusion pipettes are mounted on the perfusion chamber in a 45° angle in the path of electrodes movement. Software can control electrodes to stop at the center of each barrel for solution change. The time it takes to move between the center of the barrels (700 microns) is 140 ms. For faster solution change, people can pull 3-barrel glass pipettes to smaller size.

A small cone shaped well confines the oocyte to a fixed position. Two confined locations are designed for cell suspensions. Cultured cells on cover slip can be put on the flat bottom of the dish. A suction tube is isolated from the cells so that solution vibration from suction will not affect the oocyte.

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Units: EA


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