A system for oocyte and cell patch clamp along with two-electrode voltage clamp.
This patent-pending design is based on the PatchMAX 100A, but it comes with two computer-controlled manipulators. With the additional micromanipulator, the system can do two patch clamp experiments simultaneously, or one two-electrode voltage clamp experiment. With this new design, the system increases efficiency, decreases equipment cost, and saves space. As the system is controlled by the computer, the experiment becomes less labor intensive.
Run two patch clamp experiments simultaneously:
The ChannelMAX 100A Mini comes with two computer-controlled manipulators. As a result users can run two patch clamp experiments simultaneously. Doing two experiments at the same time allows for better decision making while doing experiments. Single channel activities are random events. It is difficult to understand what is going on by looking at the channel open and close, especially when you are working on a new channel. For example, it is hard to distinguish a channel with two open stages from two channels in the same patch. With two patches that are treated with the same conditions you have more information that could be critical in making the right decisions while working on the precious seals.
High quality patch clamp:
As in the PatchMAX 100A, the ChannelMAX 100A patch clamp is noise free and low drift. The computer controlled system eliminates human errors so the success rate of making gigaohm seals using the ChannelMAX 100A is over 90%.
Run two independent patch clamp experiments:
It is very easy to change the configuration to run two independent experiments. As shown in the figure on the right, you can easily have two perfusion chambers on the same setup.
Automated two-electrode voltage clamp:
For the two-electrode voltage, impalement is controlled by the computer so no microscope is needed. When the electrode enters the oocyte, the membrane potential changes and the movement is stopped. The user can define the membrane potential threshold and delay for the stop after the electrode enters the oocyte.
Save on equipment cost:
This system is more cost effective than a traditional electrophysiology setup. In order to reach the same functionality and productivity using a traditional setup, the equipment cost would be much higher, plus labor cost is additional. Included with the lower cost is also better quality data.
As the system can be used for both two-electrode voltage clamp and patch clamp, less lab space is required. You do not need two data acquisition systems. All the data is on one computer, so it is easy for you to compare.
Switching between patch clamp and two-electrode voltage clamp is easy:
The system is designed so that it can switch from patch clamp to a two-electrode voltage clamp easily. With all of the adjustable parts, the system can easily be adapted to different glass electrode lengths and sizes, and different amplifiers.
Use with traditional two-electrode voltage clamp experiments:
It is possible to use the ChannelMAX 100A Mini with a traditional two electrode voltage clamp to run dual two-electrode voltage clamp experiments simultaneously.
High efficiency, less labor-intensive:
As the system is automated, it is easy to run experiments in parallel to increase efficiency. Patch clamp and two-electrode voltage clamp should not be as labor-intensive as before. Let the equipment do the hard work for you!
• Two micro-manipulators, each one includes a motorized linear stage and a manual XY stage
• One perfusion chamber I and platform
• One PCI data acquisition board with BNC interface
• Mechanical error: < ± 0.05 mm
• Dimension: 8 in x 12 in x 9.5 in
• Weight: 10 lbs.
• Line voltage: 100 VAC to 120 VAC, or 220 VAC to 240 VAC
• Travel: 28 mm
• Resolution: 0.05 µm
• Maximum speed: 4 mm/s
• Lowest speed: 0.5 µm/s
• Serial/USB interface
Manual XY Stage:
• Modular Dovetail Linear Stages
• Set, lock, and forget; less susceptible to shock and vibration
• 0.5 in travel
Data Acquisition Board:
• Sixteen analog inputs, 16-bit, 250 kS/s, Input Impedance: > 10 GΩ in parallel with 100pF
• Two 16-bit analog outputs, 740 kS/s per channel. Output impedance: 0.2 Ω
• 24 digital I/O lines, 32-bit counters; digital triggering
• Analog input range: ± 10 V
• Analog output range: ± 10 V
Accepts 1/16" tubing
For full web page, please see http://www.autom8.com/auto-ch-mini.html
Download a User's Manual at http://www.autom8.com/pdfs/automatedpatch/Manual-NeoBiosystems-FAQ.pdf
Download a brochure at http://www.autom8.com/pdfs/automatedpatch/ChannelMAX-100A-Mini-Twin.pdf